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KMID : 0881720220370010015
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Journal of Food Hygiene and Safety
2022 Volume.37 No. 1 p.15 ~ p.20
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Comparison of Conventional Culture Method, Enzyme Immune Method, and PCR for the Rapid Detection of Salmonella spp. in Pet Food
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Yun Hye-Jeong
Cha Sun-Ho
Lee Seung-Hwa
Jeong Min-Hee
Na Tae-Woong
Kim Hae-Jin
Cho Hyun-Jeong
Hong Seong-Hee
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Abstract
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The purpose of this study was to compare the conventional culture method, enzyme immunemethod and the PCR method using species-specific primer in the analysis on the Salmonella spp. found in domesticallydistributed pet foods. For the study, Salmonella spp. were detected from 175 samples. From the conventionalculture method and the PCR method, two samples (jerky and corn gluten) were determined as positive. Also, from theenzyme immune method, one sample (corn gluten) was test-positive. The study revealed that application of the PCR methodwith species-specific primer allows better distinguishment between the species of the strain collected from the samples than theconventional culture method and/or the enzyme immune method.
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KEYWORD
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Salmonella, Pet food, Rapid detection, PCR
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